Recombinant Human PDGF-BB
- Cat. No.：EPT197
- Price：Negotiable
- Clicks：1120
Product Detail
Basic Information
| Size | 10ug |
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| Expression Host | E.coli |
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| Tag | NA |
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Physical Properties
| Molecular Weight | 12.42 KDa |
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| Apparent Molecular Weight | 14 KDa, reducing conditions |
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| Form | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. |
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| Purity | Greater than 98% as determined by reducing SDS-PAGE. |
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Activity & Endotoxin
| Endotoxin Level | Less than 0.02 ng/µg (0.2 EU/µg) as determined by LAL test. |
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| Activity | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
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Storage & Shipping
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| Shipping Condition | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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Detailed Description
| Background | Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. |
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