Recombinant S. hygroscopicus BAR
- Cat. No.：EPT071
- Price：USD $ 3,780.00
- Clicks：1460
Product Detail
Basic Information
| Size | 50ug |
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| Expression Host | E.coli |
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| Tag | NA |
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Physical Properties
| Molecular Weight | 20.6 KDa |
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| Apparent Molecular Weight | 18-20 KDa, reducing conditions |
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| Form | Lyophilized from a 0.2 μm filtered solution of 12.5mM Tris-HCl, 50mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2mM DTT, 1mM EDTA, pH8.5. |
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| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
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Activity & Endotoxin
| Endotoxin Level | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
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Storage & Shipping
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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| Shipping Condition | The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
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Detailed Description
| Background | Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications. |
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