Recombinant Rat VEGF 164
- Cat. No.：EPT063
- Price：Negotiable
- Clicks：716
Product Detail
Basic Information
| Size | 10ug |
|---|---|
| Expression Host | P.Pichia |
| Tag | NA |
Physical Properties
| Molecular Weight | 19.2 KDa |
|---|---|
| Apparent Molecular Weight | 18-23 KDa, reducing conditions |
| Form | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Activity & Endotoxin
| Endotoxin Level | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
|---|---|
| Activity | Immobilized Rat VEGF 164(Cat#CJ96) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc(Cat#C01A).The ED50 of Mouse VEGFR2-Fc(Cat#C01A) is 22.17 ng/ml. |
Storage & Shipping
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
|---|---|
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping Condition | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Detailed Description
| Background | Vascular endothelial growth factor (VEGF/VEGF-A ) is originally known as vascular permeability factor (VPF). It belongs to the PDGF family with a cysteine-knot structure comprised of eight conserved cysteine residues, and reckoned as a potent mediator in the process of angiogenesis and vasculogenesis in either fetus or adult. VEGF is particularly expressed in supraoptic , paraventricular nuclei and the choroid plexus of the pituitary, and abundant in the corpus luteum of the ovary and in kidney glomeruli. The rat VEGF protein contains a putative 20 amino acids (aa) signal peptide, and alternative splicing of rat VEGF gene produces isoforms of 120, 144, 164 and 188 aa. Rat VEGF164 respectively displays 97% and 88% aa identity with that regions of mouse and human VEGF. VEGF can bind to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin, and play important roles in inducing endothelial cell proliferation, promoting cell migration, inhibiting apoptosis and inducing permeabilization of blood vessels. |
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