Recombinant Human IL-33
- Cat. No.：EPT054
- Price：USD $ 1,800.00
- Clicks：1184
Product Detail
Basic Information
| Size | 10ug |
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| Expression Host | E.coli |
| Tag | NA |
Physical Properties
| Molecular Weight | 18.1 KDa |
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| Apparent Molecular Weight | 18 KDa, reducing conditions |
| Form | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Activity & Endotoxin
| Endotoxin Level | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
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| Activity | Immobilized Mouse ST2-His(Cat#CK89) at 5μg/ml (100 μl/well) can bind Human IL-33(Cat#C091).The ED50 of Human IL-33(Cat#C091) is 0.63 ug/ml. |
Storage & Shipping
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping Condition | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Detailed Description
| Background | Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length. |
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