Recombinant Mouse IL-4
- Cat. No.：EPT035
- Price：Negotiable
- Clicks：1018
Product Detail
Basic Information
| Size | 10ug |
|---|---|
| Expression Host | E.coli |
| Tag | NA |
Physical Properties
| Molecular Weight | 13.4 KDa |
|---|---|
| Apparent Molecular Weight | 14 KDa, reducing conditions |
| Form | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Trehalose, pH 6.5. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Activity & Endotoxin
| Endotoxin Level | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
|---|---|
| Activity | Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells. The ED50 for this effect is 0.01 ng/ml. |
Storage & Shipping
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
|---|---|
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping Condition | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Detailed Description
| Background | Mouse Interleukin-4(IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four αhelix structure. IL4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL4 is primarily expressed by Th2biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
|---|
Related Products:
| Product Name | Clicks |
|---|---|
| 四烯雌酮（烯丙孕素）-BSA（或OVA）抗原 | 5323 |
| 叶酸-BSA（或OVA）抗原 | 5528 |
| 乙基香兰素-BSA（或OVA）抗原 | 5570 |
| 香兰素-BSA（或OVA）抗原 | 5338 |
| 柠檬黄-BSA（或OVA）抗原 | 5429 |
| 双氯芬酸-BSA（或OVA）抗原 | 5386 |
| 氯唑西林-BSA（或OVA）抗原 | 5491 |
| 苯唑西林-BSA（或OVA）抗原 | 5507 |
| 维甲酸-BSA（或OVA）抗原 | 5596 |
| 氨苯砜-BSA（或OVA）抗原 | 5411 |
| 氰氟草酯-BSA（或OVA）抗原 | 5298 |
| 呋喃苯胺酸（呋塞米）-BSA（或OVA）抗原 | 5052 |
| 氟节胺-BSA（或OVA）抗原 | 5427 |
| 氟米松-BSA（或OVA抗原） | 5288 |
| 萘普生-BSA（或OVA）抗原 | 5604 |
| 苯菌灵-BSA（或OVA）抗原 | 5517 |
| 噻菌灵-BSA（或OVA）抗原 | 5536 |
| 三氯噻嗪-BSA（或OVA）抗原 | 5556 |
| 五氯酚酸钠-BSA（或OVA）抗原 | 5644 |
| 甲苯达唑-BSA（或OVA）抗原 | 5753 |