Product Detail

基本信息

Size	500ug
Expression Host	E.coli
Tag	NA

物理性质

Molecular Weight	49.4kDa
Apparent Molecular Weight	50-60kDa, reducing conditions
Form	Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 10 % glycerol, 1mM EDTA，pH 8.0
Purity	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Activity	Immobilized 2019-nCoV Nucleocapsid Protein-His(Cat#DRA31)at 2μg/ml (100μl/well) can bind 2019-nCoV NP Antibody (6G9)(Cat#DA027). The ED50 of 2019-nCoV NP Antibody (6G9)(Cat#DA027) is 5-30 ng/ml.

储存与运输

Storage	Reconstituted protein solution should be stored at ≤ -20°C.
Shipping Condition	The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.

详细说明

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.