Product Detail

基本信息

Size	10ug
Expression Host	Human Cells
Tag	C-Fc

物理性质

Molecular Weight	52.2 KDa
Apparent Molecular Weight	70-90 KDa, reducing conditions
Form	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Purity	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Endotoxin Level	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Activity	Immobilized Anti-Human B7-H4 mAb(Cat#NC054) at 2μg/ml (100 μl/well) can bind Mouse B7-H4-Fc(Cat#CS04).: Biotinylated by NHS-biotin prior to testing The ED50 of Mouse B7-H4-Fc*(Cat#CS04) is 4.11 ng/ml.

储存与运输

Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

Background

Mouse V-set domain-containing T-cell activation inhibitor 1/VTCN1/B7-H4 is glycosylated member of the B7 family of immune co‑stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.