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Recombinant Human EIF1AX (N-6His)

货号:EPT142
  • Price:Negotiable
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Product Detail

基本信息

Size10ug
Expression HostE.coli
TagN-6His

物理性质

Molecular Weight18.6 KDa
Apparent Molecular Weight22 KDa, reducing conditions
FormLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
PurityGreater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Endotoxin LevelLess than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

储存与运输

ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
StorageLyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping ConditionThe product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

详细说明

BackgroundEukaryotic Translation Initiation Factor 1A, X-Chromosomal (EIF1AX) is an essential eukaryotic translation initiation factor that belongs to the eIF-1A family. EIF1AX is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA and has been shown to interact with IPO13. EIF1AX contains one S1-like domain and seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.