Product Detail

基本信息

Size	50ug
Expression Host	Human Cells
Tag	N-6His

物理性质

Molecular Weight	31.5 KDa
Apparent Molecular Weight	30-40 KDa, reducing conditions
Form	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Purity	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Endotoxin Level	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

储存与运输

Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

Background

Langerin (CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis. Langerin is necessary and sufficient for Birbeck granule formation. Human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively. Trimerization greatly increases the lectin binding affinity. Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions. Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae.