Product Detail

基本信息

Size	10ug
Expression Host	E.coli
Tag	NA

物理性质

Molecular Weight	17.4 KDa
Apparent Molecular Weight	17 KDa, reducing conditions
Form	Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate, 0.2% Tween 80, pH 3.0.
Purity	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Endotoxin Level	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Activity	Loaded Human IL-2RA-Fc(Cat#CJ78) on Protein A Biosensor, can bind Mouse IL-2(Cat#CK24) with an affinity constant of 0.17 uM as determined in BLI assay.

储存与运输

Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

Background

Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL2 shares 56% and 73% aa sequence identity with human and rat IL2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL2 may be a key cytokine in the natural suppression of autoimmunity.