Product Detail

基本信息

Size	10ug
Expression Host	Human Cells
Tag	C-mFc

物理性质

Molecular Weight	42.2 KDa
Apparent Molecular Weight	50-70 KDa, reducing conditions
Form	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Purity	Greater than 95% as determined by reducing SDS-PAGE.

活性与内毒素

Endotoxin Level	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Activity	Immobilized Human OX40-Fc(Cat#CK58) at 8μg/ml (100 μl/well)can bind Human OX40L-mFc(Cat#CW25).The ED50 of Human OX40L-mFc(Cat#CW25)is 18.54 ng/ml.

储存与运输

Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping Condition	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

详细说明

Background

Tumor necrosis factor ligand superfamily member 4(TNFSF4/OX40L) is a single-pass type II membrane protein.OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40-OX40L co-stimulates signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. It involved in T-cell proliferation and cytokine production. Additional, it has been found association with systemic lupus erythematosus, no association with occurrence of atherosclerosis.